Figure 2 | Scientific Reports

Figure 2

From: Differences in intracellular localisation of ANKH mutants that relate to mechanisms of calcium pyrophosphate deposition disease and craniometaphyseal dysplasia

Figure 2

P5L overexpression intracellular localisation and biochemical effects compared to wt.ANKH control in CH-8 and HEK293 cells. P5L-GFP (green) mainly localised to HEK293 cytoplasm at 24 H and cell membrane at 72 H (A); increased ePPi in HEK293 culture media (B); ARS staining of CH-8 cells (left) and concentration of ARS/µg protein (right) normalized to ActB confirming increased mineralization (C); no significant change in ENPP1 (D), significant increased TNAP at 24 H and trended increase at 72 H (E), no significant change in PIT-1 (F); ENPP1 activity in CH-8 cells increased at 24, 48 and 72 H (G). H = hours, scale bars = 20 μm; *** indicates p value < 0.01; * indicates p value < 0.05. # indicates p value < 0.1; neg. = Negligible/Raw value below threshold.

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