Figure 3
Expression profiles of MiDaf16-like1 (Minc3s02528g30466) and MiSkn1-like1 (Minc3s02028g27861) in response to oxidative stress and plant parasitism. (A) Survival percentage (%) of M. incognita J2 race 3 after overnight exposure to different concentrations of H2O2, as described by Vicente et al.61. Error bars represent confidence intervals corresponding to three biological replicates. Different letters above the columns indicate significant differences (p-value <0.05) between different survival percentages in each H2O2 treatment, according to Tukey’s test. (B) Expression profile of MiDaf16-like1 and MiSkn1-like1 in M. incognita J2 race 3 after 4 and 12 h of exposure to different concentrations of H2O2. (C) Expression profile of MiDaf16-like1 and MiSkn1-like1 during M. incognita parasitism (5 to 30 days post-inoculation; dpi) in (C) A. thaliana and (D) N. tabacum. (E) Expression profile of MiSod3-like1, MiTTL5-like1, and MiGst1-like1 during exposure of M. incognita J2 race 3 to H2O2. Newly hatched J2 were used as controls for all assays of gene expression level. Error bars represent confidence intervals corresponding to three biological replicates. (F) Expression profiles of MiDaf16-like1 and MiSkn1-like1 in different life stages of M. incognita using transcriptome datasets (BioProject number: PRJNA39055939); retrieved from the BioSample database (NCBI). Error bars represent confidence intervals corresponding to three libraries per life stage of the nematode. Expression profile measured by real-time PCR of the MiDaf16-like1 (G) and MiSkn1-like1 genes in different life stages of M. incognita race 3. The relative expression levels were normalized with the β-tubulin (MiTUB) and glyceraldehyde 3-phosphate dehydrogenase (MiGAPDH) endogenous reference genes (Supplemental Table 3). Error bars represent confidence intervals corresponding to three biological replicates.
