Figure 5

Receiver operating characteristics (ROC) curves. The AFperAMT for each tissue core was calculated using ImageJ software. AFperAMT for each core was then indexed according to the histopathological diagnosis provided independently by two veterinary pathologists evaluating a serial section of the TA stained with H&E. ROC curves were constructed using MedCalc software. Area under the curve (AUC) values and optimal sensitivity and specificity calculated using the Youden index (considered a measure of the best putative performance of a dichotomous diagnostic test) are as follows: FRA1; sensitivity = 72.34, specificity = 88.57, Area under the curve (AUC) = 0.854, criterion value > 74.1. c-Myc; sensitivity = 41.49, specificity = 91.43, AUC = 0.637, criterion value > 62.5. Cyclin D1; sensitivity = 61.70, specificity = 53.29, AUC = 0.559, criterion value > 61.5. MMP7; sensitivity = 92.56, specificity = 37.14, AUC = 0.627, criterion value <74.1.