Figure 1 | Scientific Reports

Figure 1

From: Nucleic acid recognition and antiviral activity of 1,4-substituted terphenyl compounds mimicking all faces of the HIV-1 Rev protein positively-charged α-helix

Figure 1

(A) Comparison of bilateral terphenyls with side chains in relative 1,3 (left) and 1,4 (right) positions. In both cases, the aminoalkyl side chains imitate the Arg residues of Rev. (B) Schematic overlay of a 1,4-bilaterally substituted terphenyl and a protein α-helix showing the mimicked residues (cyan). (C) Three-dimensional view of the complex formed between subdomain IIB of the RRE (grey) and the Rev34–50 helix of the HIV-1 protein Rev (yellow)12. The extrahelical loop residues A19 and U23 are coloured light green. The image was generated with MOE 2019.0102 (www.chemcomp.com). (D) Secondary structure of RNA hairpins IIBh, containing the high-affinity Rev34–50 binding site, and TARh, used as a specificity control. For fluorescence intensity assays a fluorescein probe was linked to the extra-helical loop residues U23 of IIBh and U8 of TARh (indicated with asterisks).

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