Table 1 50% inhibitory concentrations of 1,4-terphenyl molecules for RRE subdomain IIB-Rev34–50 and high-order RRE-Rev complex formation.

From: Nucleic acid recognition and antiviral activity of 1,4-substituted terphenyl compounds mimicking all faces of the HIV-1 Rev protein positively-charged α-helix

Compound

IC50, RRE subdomain IIB-Rev34–50 (μM)a

IC50, high-order RRE-Rev (μM)b

1a

14 (9.0–20, 0.8691)

6.8 (3.8–12, 0.8337)

1b

>100

>50

1c

>100

n/d

1d

47 (33–68, 0.7690)

3.0 (2.0–4.7, 0.9242)

2a

>100

n/d

2b

>100

n/d

3a

>100

n/d

3b

>100

n/d

3c

>100

>50

4

>100

n/d

  1. aRRE subdomain IIB–Rev34–50 IC50 values were obtained with fluorescence anisotropy experiments using 60 nM IIBh and 10 nM frevp in the presence of 100 mM KCl, 20 mM NaCl and 2 mM MgCl2. bFull-length RRE–Rev IC50 values were measured by EMSA with 78 nM RRE, 1.32 μM Rev, 300 mM KCl and 1 mM MgCl2, analysing the band corresponding to high-order complexes. The table shows best-fit IC50 values, with 95% confidence intervals and R2 coefficients shown in parentheses when applicable. n/d: not determined.
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