Figure 3
MS/MS spectrum of the isopeptide between VQGQGIIQPQQPAQL (γ-gliadin) and FLKNAGR (TG2). (a) Spectrum of the isopeptide annotated with fragments of the γ-gliadin peptide with TG2-peptide as modification as annotated by MQ Viewer (spectrum is shown decharged with fragments only single charged). The fragments are marked in different colours as follows: y-fragments in red, b-fragments in blue, a- and c-fragments in turquoise, fragments with losses of NH3 or CO marked in orange. (b) Spectrum of the isopeptide annotated manually with fragments of both sides of the isopeptides, calculated with ProteinProspector. The insert amplifies the range between m/z 100 to 400. The fragments are marked in different colours as follows: y-fragments of the γ-gliadin peptide in pink, b-fragments of the γ-gliadin peptide in blue, y-fragments of TG2 peptide in violet, a- and internal fragments in turquoise, fragments with losses of NH3 or CO marked in orange.
