Table 2 Isopeptides between TG2 and peptides derived from rye gluten protein types.

Abb.^a	TG2 lysine^b	Gluten peptide^c	GPT^d	UniProtKB accession	UniProtKB name	Organism	MQ score^e	Fragments (discovery)^f		Manually checked (discovery)^g		Fragments (targeted)^h
Abb.^a	TG2 lysine^b	Gluten peptide^c	GPT^d	UniProtKB accession	UniProtKB name	Organism	MQ score^e	α ⁱ	β^j	α	β	α	β
R1^l	K205	IVQGQSIIQQQPAQL	γ40k	H8Y0N7	gamma prolamin	S. cereale ssp. afghanicum	68.97	17	4	34	4	13	3
R2^l	K429	AQVQGIIQPQQL	γ75k	A4GU91	75k gamma secalin	S. sylvestre	59.25	9	—	10	3	12	—
R3^l	K600	QPQQPFPQQPQQSF	γ75k	H8Y0K1	gamma prolamin	P. juncea	80.75	14	—	39	2	10	—
R4^l	K677	AQVQGIIQPQQL	γ75k	A4GU91	75k gamma secalin	S. sylvestre	90.05	16	2	36	5	19	2
R5	K677	QIPTPLQPQQPF	ω	Q41210	C-hordein	H. vulgare	57.18	14	1	41	3	10	1
R6	K677	AQIPQHL	γ40k	H8Y0N7	gamma prolamin	S. cereale ssp. afghanicum	62.98	10	3	24	8	9	3

^aAbb., abbreviation, ^bLysine residue in the TG2 sequence, K205: peptide FLKNAGR, K429: ISTKSVGR, K600: QKR, K677: AVKGFR, ^cGlutamine residues involved in crosslinking to TG2 are highlighted in bold, deamidation sites underlined, ^dGPT, gluten protein type, γ40k, γ-40k-secalins, γ75k, γ-75k-secalins, ω, ω-secalins, ^eMQ, MaxQuant, ^fNumber of fragments identified by discovery-driven nLC-MS/MS and MaxQuant data analysis, ^gNumber of fragments identified by discovery-driven nLC-MS/MS and manual inspection of full scan spectra considering additional internal fragments calculated by ProteinProspector, ^hNumber of fragments identified by PRM analysis, ⁱα, α-side of the isopeptide (gluten peptide), ^jβ, β-side of the isopeptide (TG2 peptide), ^lCrosslinking site identified by PRM analysis.

Quick links

Search