Figure 3
Glutamate increases in several brain regions in Scrapper-knockout (SCR-KO) mice. (a) Hematoxylin and eosin (HE) stained brain sections of wild type (WT) and SCR-KO mice. Signal intensity of Glu in sagittal brain sections is shown on a rainbow-color scale (scale bar, 2 mm). Several brain regions of WT and SCR-KO mice were selected for detailed analysis and are presented as histograms of grayscale images (scale bar, 2 mm). (b) The ratios of the glutamate signal intensity in each brain region between the SCR-KO and WT mice. The SCR-KO/WT intensity ratio of the geometric mean of the signal intensity histogram in each comparison is represented as a point (n = 5 mice); the geometric mean approximates to the peak of the histogram. The arithmetic mean and standard deviation of SCR-KO/WT intensity ratios is displayed as a bar. Asterisks indicate brain regions that featured significant differences (*p < 0.05) in the student’s t test between the SCR-KO/WT intensity ratio and the null distribution, which has a mean of 1 and the same standard deviation as that of the geometric mean distribution of each brain region.
