Figure 1
From: Polymorphisms in miRNA binding sites involved in metabolic diseases in mice and humans
Conceptual overview of the study design (A) and workflow (B) for the identification of polymorphisms in miRNA-mRNA binding sites. (a) (1) Identification of genomic variants in miRNA-mRNA binding sites. (2-3) Comparison of genes with an impaired miRNA binding site with genes that have higher expression levels in tissues of NZO mice. (4) Conservation of polymorphisms between mice and humans. (5) Scanning for metabolically relevant genes that have an impaired miRNA binding site and a conserved polymorphism. (6) Enrichment analysis of results. (b) (1) miRNAs expressed with a readcount >5 according to miRBase22 were filtered. (2) Results of a genome-wide screen for miRNA-mRNA binding sites as predicted by the miRanda tool. (3) Reduction of the number of possible miRNA-mRNA-interactions by using several prediction tools. (4) Identification of SNPs within miRNA-mRNA binding sites. (5) Comparison of genes carrying SNPs within miRNA-mRNA binding sites with transcriptome data of brown adipose tissue (BAT), gonadal white adipose tissue (gWAT), skeletal muscle and liver. (6) Venn diagram illustrating the number of genes located in QTL per tissue exhibiting higher expression levels and a SNP in a miRNA-mRNA binding site. Tissue images adapted from: https://smart.servier.com/.
