Figure 1

Impact of sc1o on cell viability, mitochondrial toxicity and cell cycle. (a) For the cell viability assay, HCT 116 cells were incubated with 0.01–50 µM of sc1o over 24 h. To calculate cell viability, the absorbance of DMSO-treated cells was set to 100%, and the sc1o samples were correlated to the absorbance of DMSO value. (b) For the apoptosis assay, HCT 116 cells were incubated with 0.01–50 µM of sc1o over 24 h. To calculate the apoptotic rate, the number of apoptotic cells were correlated to the total number of cells. (c) Dose response curves for mitochondrial toxicity by sc1o. The MitoTracker Red CMXRos dye was used to stain mitochondria in live cells. Analysis of mitochondrial toxicity was performed with the Opera imaging system. Sc1o was profiled in the assay in 11-point concentration–response format with the raw data normalized using the positive control (1 µM valinomycin) and negative control (DMSO). (d) For cell cycle analysis, HCT 116 cells were incubated with sc1o in concentration as indicated or with controls over 24 h. Using flow cytometry, the amount of cells in the various cell cycle phases (G1, S, G2) was determined. The flow cytometry data was analysed with a specific cell cycle analysis method from FlowJo software. For statistical analysis, one-way (a, b) or two-way (d) ANOVA with Dunnett’s comparison tests were used. The experiments were performed in triplicate and repeated three times. *p < 0.05, ***p < 0.001 indicate significant differences between sc1o and DMSO data.