Figure 3 | Scientific Reports

Figure 3

From: An Ex Vivo Brain Slice Culture Model of Chronic Wasting Disease: Implications for Disease Pathogenesis and Therapeutic Development

Figure 3

The anti-prion activity of compounds and the capture of CWD prions from biological samples using slice cultures. (A) The anti-prion activity of the compounds astemizole, Congo red, and quinacrine was evaluated against a CWD-infected control and 5 ng, 0.5 ng, 50 pg serial log dilutions. Inhibitory activity depended on the seed dilution, except for astemizole, which had no apparent effect on the seeding activity of CWD prion-infected slice cultures. (B) RT-QuIC assay showing transmission of CWD prions from infected recto-anal mucosa-associated lymphoid tissue (RAMALT) samples to the slices cultured with RAMALT homogenates for 21 days when tested at log-fold dilutions of 50 ng to 50 pg. A CWD-infected RAMALT homogenate was used as a positive control, while a healthy RAMALT control homogenate served as a negative control. Each RT-QuIC trace represents the average fluorescence reading from 3 technical replicates of slice cultures.

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