Figure 1 | Scientific Reports

Figure 1

From: Salivary proteome signatures in the early and middle stages of human pregnancy with term birth outcome

Figure 1

Schematic representation of the label-free quantitative discovery and MRM based targeted validation workflow. The label-free DIA approach with a saliva sample (N = 20) was taken to evaluate saliva proteome along with saliva-specific spectral library generation by DDA method. The DDA data were exported to MaxQuant for library generation and subsequently, each DIA file was analyzed with a prebuilt saliva spectral library in Spectronaut. An independent set of saliva samples (N = 14) was used to corroborate predicted central regulator proteins by MRM. More details can be found under Material and Methods.

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