Figure 2
From: Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies
Photos of individual steps during the purification of LDCVs. (a) Adrenal glands transported on ice from local slaughterhouse. Note that adrenal glands are surrounded by fat. (b) Adrenal medullae after removal of adrenal cortex. (c) Homogenate (H) of adrenal medullae after homogenization. (d) Supernatant (S1) and pellet (P1) after centrifugation. (e) P2 fraction before and, (f) after washing. (g) LDCV distribution after continuous sucrose gradient. LDCVs are pink, which results from the presence of cytochrome b561 in the LDCV membrane27, whereas mitochondria are brown. Note that the pellet is pink and the supernatant is brown. (h) After removing the supernatant, pellet are LDCVs.
