Figure 5
From: Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies
Detection of miR-375 stored in LDCVs. (a) Schematic of test for miR-375 stored in LDCVs (top); 1% (vol/vol) TX-100 was incubated with LDCVs to disrupt the vesicle membrane. RNase A was then applied to degrade miR-375. Relative levels of miR-375 with or without 1% (vol/vol) TX-100 were determined by qRT-PCR (bottom). (b) LDCVs were treated with Proteinase K in the presence of TX-100, followed by RNase A treatment. (c) RNase A was applied after RNA extraction using the miRNeasy Mini Kit. Proteins are removed from RNA samples. Values represent a percentage of control and data are mean ± SD from three to four independent replicates.
