Table 1 Tests for the stability of miR-375 stored in LDCVs.
From: Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies
Treatment Reactions | Control | +RNase A | +TX-100 + RNase A | +TX-100 + Proteinase K + RNase A | ||||
|---|---|---|---|---|---|---|---|---|
Vol. | Final Conc. | Vol. | Final Conc. | Vol. | Final Conc. | Vol. | Final Conc. | |
LDCVs | 5 µl | 50 µg | 5 µl | 50 µg | 5 µl | 50 µg | 5 µl | 50 µg |
Syn-cel-miR-39 | 5 µl | 0.5 pmol | 5 µl | 0.5 pmol | 5 µl | 0.5 pmol | 5 µl | 0.5 pmol |
RNase-DNase Free PBS | 10 µl | 7.5 µl | 5.5 µl | 6 µl | ||||
10% TX-100 (vol/vol) | 2 µl | 1% | 2 µl | 1% | ||||
Proteinase K(1 mg/ml) | 2 µl | 100 µg/ml | ||||||
Protease inhibitor (2×) | 10 µl | |||||||
RNase-DNase Free PBS | 5 µl | |||||||
RNase A (100 µg/ml) | 2 µl | 10 µg/ml | 2 µl | 10 µg/ml | 4 µl | 10 µg/ml | ||
RNase inhibitor (40 U/µl) | 0.5 µl | 1 U/µl | 0.5 µl | 1 U/µl | 1 µl | 1 U/µl | ||
Final Volume | 20 µl | 20 µl | 20 µl | 40 µl | ||||
