Figure 1 | Scientific Reports

Figure 1

From: Serum alpha-mannosidase as an additional barrier to eliciting oligomannose-specific HIV-1-neutralizing antibodies

Figure 1The alternative text for this image may have been generated using AI.

PGT128 and related bnAbs bind the CRM197-conjugated mimetic (NIT211) with similar or greater avidity as the BSA-conjugated version (NIT82B). The NIT211 derivative (NIT211_3) used here is loaded at 3.5 glycosides per CRM197. NIT82B is loaded at 4.4 glycosides per BSA. The conjugates (63–73 kDa) were coated as solid-phase antigen onto microtiter-plate wells at 5 µg/ml and assayed for recognition by PGT125, 126, 128, and 130. All antibodies were tested as IgGs.

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