Figure 2 | Scientific Reports

Figure 2

From: Serum alpha-mannosidase as an additional barrier to eliciting oligomannose-specific HIV-1-neutralizing antibodies

Figure 2The alternative text for this image may have been generated using AI.

Serum mannosidase trims protein-conjugated oligomannose mimetic in vitro. Shown is PGT128 binding to the BSA-conjugated (a) or the CRM197-conjugated glycoside (b) (5 µg/ml) after in situ overnight incubation of glycoconjugate-coated ELISA plates with buffer, mammalian serum, serum supplemented with kifunensine (Kif) or serum supplemented with EDTA. All experiments for a given serum were performed on a single assay plate to avoid potential plate-to-plate variability. Shown are representative results from two independent experiments.

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