Figure 4 | Scientific Reports

Figure 4

From: Transcriptional profiling of murine macrophages stimulated with cartilage fragments revealed a strategy for treatment of progressive osteoarthritis

Figure 4

Identification of macrophage receptors to cartilage fragments and functional blocking assay. (A) A predicated model of macrophage activation by cartilage fragments based on bioinformatic analysis. (B) Detection of MARCO, TLR2 and ITGα5 in synovial tissues of OA patients. Representative images form sections of synovial tissues of 8 OA patients. Lower panels show section stained by rabbit IgG and mouse IgG2 as negative controls. Scale bars are indicated on each image. (C) Effects of functional blocking antibodies to MARCO, TLR2 and ITGα5 on the secretion of TNF-α. Macrophages were pretreated with 10 µg/ml for 30 min and then cultured with cartilage fragments. Results are presented as means ± standard errors of the means from 4 wells values for each treatment. Significant difference was determined by one-way ANOVA followed by Tukey’s multiple-comparison procedure. The results are representative of two independent experiments. Experiments were repeated twice for reproducibility of data.

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