Figure 2
From: On the use of DNA as a linker in antibody-drug conjugates: synthesis, stability and in vitro potency
(A) Structures of ADC T-MMAE and T-DNA-MMAE. (B) Denaturing SDS PAGE (4–15%) analysis showed complete hybridization of T-DNA-MMAE and no sign of unconjugated payloads (bands of DNA-MMAE and ON-Cy5 served as a positive control; the cropped parts of the same gel are displayed for the fluorescent gel, for the full-length gel see SI Figure S12). (C) Normalized UV-Vis spectra of the conjugates demonstrated successful conjugation of about two payloads per trastuzumab (average DoCUV = 2.1, see SI Table S1). (D) Deconvoluted native MS of the ADCs.
