Figure 2 | Scientific Reports

Figure 2

From: Expansin-like Exl1 from Pectobacterium is a virulence factor required for host infection, and induces a defence plant response involving ROS, and jasmonate, ethylene and salicylic acid signalling pathways in Arabidopsis thaliana

Figure 2

Expansin expression correlates with the level of infection. (A) P. brasiliense is more virulent than P. atrosepticum on potato tuber causing higher tissue maceration at 7 dpi/23 °C. Box plots (showing median and first and third quartiles and whiskers indicating the range of outliers) of three independent experiments (n = 30); significance p < 0.01 (*) was calculated with the Mann-Whitney test. (B) Western blot of the over-expression (OE) of exl1 gene P. brasiliense BF45 compared to the untransformed strain (UT), and control of total protein (TP). Complete membranes with different contrasts can be found in the Supplementary materials. (C) Increased celery tissue maceration by exl1 over-expression (OE) in P. brasiliense BF45 in comparison to untransformed P. brasiliense (UT) and to GFP overexpression as an irrelevant control (C). Box plots of three independent experiments (n = 30); significance p < 0.0001 (****) was calculated with Kruskall-Wallis tests for non-parametric data.

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