Figure 6 | Scientific Reports

Figure 6

From: Expansin-like Exl1 from Pectobacterium is a virulence factor required for host infection, and induces a defence plant response involving ROS, and jasmonate, ethylene and salicylic acid signalling pathways in Arabidopsis thaliana

Figure 6

Exl1 infiltration triggers a burst of reactive oxygen species. (A) Relative expression level of ROS marker gene ZAT12 by RT-qPCR. (B,C) Reactive oxygen species detected with dichloro-dihydro-fluorescein diacetate (DCFH-DA) in A. thaliana leaves 30 min (B) and 90 minutes (C) post infiltration with the indicated buffer (Mock), or 3.7 μM proteins: Exl1, B. subtilis EXLX1 and the Exl1 triple mutant Y125A/W126A/Y157A (YWY). Fluorescent areas were determined using the Fiji software; FU are fluorescence units in pixels/cm2. Box plots of at least three independent experiments (n = 12); significances p < 0.001 (**) and p < 0.00001 (****) were calculated according to an ordinary one-way ANOVA.

Back to article page