Figure 7 | Scientific Reports

Figure 7

From: Expansin-like Exl1 from Pectobacterium is a virulence factor required for host infection, and induces a defence plant response involving ROS, and jasmonate, ethylene and salicylic acid signalling pathways in Arabidopsis thaliana

Figure 7

Exl1 activity triggers a plant defence response involving JA, ET and SA pathways in A. thaliana leaves. Normalised RNA expression levels by RT-qPCR of marker genes involved in the biosynthesis of plant defence hormones: jasmonic acid, PDF1.2 (A) and AOS (B); ethylene, PR4 (C); salicylic acid, PR1 (D) and EDS5 (E). Expression was analysed 5 min, 1 h and 24 h after buffer (Mock) or 3.7 μM Exl1 infiltration, and again 24 h after a challenge with P. brasiliense (Pb) previously treated with Exl1 for 24 hours. (F) A. thaliana mutants impaired in the synthesis of JA (jar-1) and SA (NahG and eds5) previously treated (for 24 h) with Exl1 fail to confer protection and become more susceptible towards P. brasiliense BF45. Error bars indicate the +/− standard deviations of the average across three independent experiments (n = 70); no significant differences were determined with a Mann-Whitney test for non-parametric data.

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