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Figure 2

From: High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture

Figure 2

Example of a BsAb purified using the DNA imbalance + CH1 purification method. (A) Analytical SEC chromatogram of the initial protein A eluate featuring a main peak comprised of target heterodimer and scFv-Fc homodimer in addition to a minor peak comprised of scFv-Fc half Ab. (B) Analytical SEC chromatogram of the CH1 eluate from (A) showing capture of only the desired heterodimeric BsAb. (C) Non-reduced GXII analysis of the protein A eluate (lane 1) and the CH1 eluate (lane 2). Captured species are indicated on the gel image.

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