Figure 2 | Scientific Reports

Figure 2

From: A novel functional cross-interaction between opioid and pheromone signaling may be involved in stress avoidance in Caenorhabditis elegans

Figure 2

The role of opioid-like signaling in ascaroside pheromone-induced physiology. (a) The amount of ascaroside pheromone biosynthesis in the N2 wildtype, nlp-24(tm2105), npr-17(tm3210), and daf-22(ok693) as a negative control (n = 3). The data are represented as the mean with SEM from more than three different experiments with three technical repeats. *, **, and *** indicate p-values <0.05, <0.01, and <0.001, respectively. N.S. indicates no statistically significant difference. (b,c) The expression of NLP-24::GFP in the ASI neurons under fed, STS, LTS, and with ascr#2 conditions in the npr-17(tm3210) background (b) at 20°C, (c) at 25°C. Method and data processing for measured values are the same as in the legend of Fig. 1. Each box in the box plot was represents the inter-quartile range, the bar in the box represents median, and the X symbols represents the mean of the measured values under each condition. N.S. indicates no statistically significant difference. All p-values were calculated by unpaired t-test.

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