Figure 3

The relationship between opioid signaling and the ascaroside pheromone receptor in the ASI neurons. (a–c,f) The dauer formation ratios of (a) the nlp-24(tm2105) and npr-17(tm3210) worms (n = 5), (b) the nlp-24-overexpression lines, (n = 3), (c) the npr-17-overexpression lines (n = 4), and (f) the double transgenic line expressing npr-17 and daf-37 specifically in the ASI neurons. The dauer formation assay was performed by incubating eggs from each strain on plates containing 38 μM ascr#2 (>95.0% pure by HPLC) at 25°C for 3 days and then counting the dauer larvae. The data are represented as the mean with SEM from more than three different experiments with three technical repeats. *, **, and *** indicate p-values <0.05, <0.01, and <0.001, respectively. N.S. indicates no statistically significant difference. (d,e) The expression of NLP-24::GFP in the ASI neurons under fed, STS, and with ascr#2 conditions in the ASI-specific daf-37 overexpression line (d) at 20 °C, and (e) at 25°C. Method and data processing for measured values are the same as in the legend of Fig. 1. Each box in the box plot was represents the inter-quartile range, the bar in the box represents median, and the X symbols represents the mean of the measured values under each condition. N.S. indicates no statistically significant difference. All p-values were calculated by unpaired t-test.