Figure 6

The effects of the skn-1 and TGF-β pathways on opioid-like signaling. (a–f) The expression of NLP-24::GFP in the ASI neurons under fed, starved, and starved with ascr#2 conditions in the skn-1(zj15) background (a) at 20°C, (b) at 25°C, (c) in the daf-7(e1372) background at 15°C, (d) at 25°C, (e) in the nhr-69(ok1926) background at 20°C, and (f) at 25°C. Method and data processing for measured values are the same as in the legend of Fig. 1. Each box in the box plot was represents the inter-quartile range, the bar in the box represents median, and the X symbols represents the mean of the measured values under each condition. *, **, and *** indicate p-values <0.05, <0.01, and <0.001, respectively, compared to the fed condition. #, ##, and ### indicate p-values <0.05, <0.01, and <0.001, respectively, compared to the STS. x, xx, and xxx indicate p-values <0.05, <0.01, and <0.001, respectively, compared to STS with ascr#2 treatment (100μM). ^, ^^, and ^^^ indicate p-values <0.05, <0.01, and <0.001, respectively, compared to the LTS condition. N.S. indicates no statistically significant difference. All p-values were calculated by unpaired t-test. (g) The dauer formation ratio of the nlp-24(tm2105);nhr-69(ok1926) double mutant worms (n = 3). The method of dauer formation assay are same as in the legend of Fig. 3. The data are represented as the mean with SEM of three different experiments with three technical repeats. *, **, and *** indicate p-values <0.05, <0.01, and <0.001, respectively. N.S. indicates no statistically significant difference. All p-values were calculated by unpaired t-test.