Table 2 Kinetic parameters of Z. jujuba peroxidase.

From: A novel peroxidase from Ziziphus jujuba fruit: purification, thermodynamics and biochemical characterization properties

Parameters

Guaiacol

H2O2

Km (mM)

23.47

35.5

Vmax µmole ml−1 min−1

0.23

0.91

Kcat (S−1)

588

1538

Kcat/Km

25

43

  1. One milliliter of a reaction mixture containing 0.05 M sodium acetate buffer (pH 5.5), suitable amount of enzyme and concentrations of guaiacol ranging from 20 to 90 mM. and hydrogen peroxide ranging from 4 to 12 mM. The absorbance was recorded in 60 s-intervals for 3 min at 470 nm. Each point represents the mean of three experiments ± S.E.
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