Table 4 Substrate specificity of Z. jujuba peroxidase.

From: A novel peroxidase from Ziziphus jujuba fruit: purification, thermodynamics and biochemical characterization properties

Substrate

Z. jujube peroxidase

Guaiacol

100 ± 0.011

O-Dianisidine

67 ± 0.075

4-Aminoantipyrine

37 ± 0.016

Pyrogallol

21 ± 0.049

ABTS

14 ± 0.010

O-Phenylenediamine

87 ± 0.022

  1. One milliliter of a reaction mixture containing 0.008 M hydrogen peroxide, 0.04 M substrate, 0.05 M sodium acetate buffer (pH 5.5), and an amount of enzyme. The absorbance was recorded in 60 s-intervals for 3 min at 470 nm. Each point represents the mean of three experiments ± S.E.
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