Table 5 Effect of various metal ions and chemical compounds on the activity of the purified Z. jujuba peroxidase.

From: A novel peroxidase from Ziziphus jujuba fruit: purification, thermodynamics and biochemical characterization properties

Metal ion and/or chemical compounds

Concentration

Relative activity %

control

—

100 ±  1.24

Cu2+

5 mM

121  ± 1.29

Fe2+

96  ± 1.42

Cd2+

9  ± 0.02

Ni2+

56  ± 0.18

Zn2+

13 ± 1.86

Hg2+

5 ± 0.03

Ca2+

108  ± 1.33

EDTA

89 ± 0.56

β-mercaptoethanol

0.0 ± 0.00

NaN3

27 ± 0.013

SDS

88 ± 0.41

Urea

2 M

69 ± 1.42

Triton X-100

5%

73 ± 0.45

Isopropanol

5%

96 ± 0.33

  1. One milliliter of a reaction mixture containing 0.008 M hydrogen peroxide, 0.04 M guaiacol, 0.05 M sodium acetate buffer (pH 5.5), different concentration of metal ions and/or organic solvents and an amount of enzyme. The absorbance was recorded in 60 s-intervals for 3 min at 470 nm. Each point represents the mean of three experiments ± S.E.
Back to article page