Figure 3
Performance of mcr−9 LAMP assay using qLAMP and eLAMP. (A) Output from qLAMP. Top panel shows the normalized negative (n = 87) and positive (n = 41) amplification curves in orange and blue, respectively. Bottom panel shows the distribution of qLAMP TTP for positive isolates. (B) Algorithm to extract amplification curve and TTP from LoC ISFET array output. (C) Output from eLAMP. Top panel shows the normalized negative (n = 6) and positive (n = 14) amplification curves in orange and blue, respectively. Bottom panel shows the distribution of eLAMP TTP for positive isolates. TTP was extracted using a threshold at 10% of the maximum. See Figures S1–S4 and Table S4 for detailed information.
