Figure 2
An inhibitor of SFKs, PP2, prevents the reduction of LynA accompanying the depletion of Csk. HCT116 cells were transfected with siRNA for Csk or the control siRNA and then cultured for 48 h. During the last 24 h of the total 48 h culture, cells were incubated in medium containing 10 μM PP2 or DMSO (solvent control) and analysed by Western blotting with antibodies against the indicated proteins. The numbers on the right side of the p-Tyr panel indicate the electrophoretic positions of the molecular weight marker proteins. The letters ‘S and Y’ on the right side of the panels for p-SFKs (A-loop) and p-Src Y530 indicate the electrophoretic position of c-Src and c-Yes. Full-length blots are presented in Fig. S8.
