Figure 4
Cbls mediate the reduction of LynA accompanying the depletion of Csk. Western blot analysis with antibodies against the indicated proteins was conducted. HCT116 (a,b) or HeLa S3 (c,d) cells were transfected with siRNA for Csk or control siRNA and then cultured for 48 h. Two siRNAs for Csk with different nucleotide sequences were used. HCT116 (e–h) or HeLa S3 (i,j) cells were transfected with a combination of siRNAs for the indicated targets and cultured for 48 h. Control cells were transfected with control siRNA (e–g and i, each lane 1). Graphs (b), (d), (h), and (j) show the average ± SD of the relative intensity of the individual bands calculated from three sets of samples (see also Fig. S2). In graphs (b) and (d), the p-value against the control was calculated by Dunnett’s test, and the asterisks and NS indicate p < 0.05 and p > 0.1, respectively. In graphs (h) and (j), the p-values between all pairs of samples were calculated by Tukey’s HSD test. Significant differences (p < 0.05) were found only between the pairs indicated by the asterisks. Full-length blots are presented in Fig. S10.
