Table 2 Comparison of microbiological diagnosis by culture, 16S rRNA PCR and targeted PCR at a London NHS laboratory network, April 2015 and April 2019.

From: Clinical utility and cost-effectiveness of bacterial 16S rRNA and targeted PCR based diagnostic testing in a UK microbiology laboratory network

Organism

Culture (n = 607)

16S rRNA PCR (n = 607)

Targeted PCR (n = 367)

Gram Positive Organism

Staphylococcus aureus

8

1

9

Unidentified Staphlococcus spp

0

2

n/a

Coagulase negative Staphyloccocus spp

17

3

n/a

Streptococcus pneumoniae

0

2

13

Group A/B Streptococcus spp

0

6

10

Streptococcus dysgalactiae

0

2

n/a

Streptococcus milleri group

2

3

n/a

Unidentified Streptococcus spp

2

6

n/a

Enterococcus sp

3

2

n/a

Propionibacterium sp

2

2

2

Bacillus sp

0

1

n/a

Corynebacterium sp

0

1

n/a

Dermacoccus sp

0

1

n/a

Eubacterium sp

0

1

n/a

Gemella sp

0

1

n/a

Aerococcus sp

1

0

n/a

Gram Negative Organism

Haemophilus sp

1

6

1

Pseudomonas sp

3

3

n/a

Escherichia coli

1

3

1

Klebsiella sp

0

1

n/a

Herbaspirillum sp

0

2

n/a

Prevotella sp

1

1

n/a

Aeromonas sp

0

1

n/a

Haematobacter sp

0

1

n/a

Neisseria sp

0

1

0

Proteus sp

0

1

n/a

Ureaplasma sp

0

1

1

Achromobacter sp

1

0

n/a

Paracoccus sp

0

1

n/a

Bartonella sp

0

0

1

Chlamydia sp

0

0

1

Kingella kingae

0

0

1

Anaerobes

Fusobacterium sp

0

3

n/a

Aggregatibacter sp

0

1

n/a

Actinomyces

1

0

0

Other

Mycoplasma sp

0

5

0

(Candida albicans)

1

0

n/a

Mycobacterium sp

0

0

3

Unidentified organism

3

3

n/a

Mixed species

4

10

n/a

Total positives

51

78

43

  1. Abbreviations: ribosomal Ribonucleic Acid (rRNA), Polymerase Chain Reaction (PCR)