Table 2 Comparison of microbiological diagnosis by culture, 16S rRNA PCR and targeted PCR at a London NHS laboratory network, April 2015 and April 2019.
Organism | Culture (n = 607) | 16S rRNA PCR (n = 607) | Targeted PCR (n = 367) |
|---|---|---|---|
Gram Positive Organism | |||
Staphylococcus aureus | 8 | 1 | 9 |
Unidentified Staphlococcus spp | 0 | 2 | n/a |
Coagulase negative Staphyloccocus spp | 17 | 3 | n/a |
Streptococcus pneumoniae | 0 | 2 | 13 |
Group A/B Streptococcus spp | 0 | 6 | 10 |
Streptococcus dysgalactiae | 0 | 2 | n/a |
Streptococcus milleri group | 2 | 3 | n/a |
Unidentified Streptococcus spp | 2 | 6 | n/a |
Enterococcus sp | 3 | 2 | n/a |
Propionibacterium sp | 2 | 2 | 2 |
Bacillus sp | 0 | 1 | n/a |
Corynebacterium sp | 0 | 1 | n/a |
Dermacoccus sp | 0 | 1 | n/a |
Eubacterium sp | 0 | 1 | n/a |
Gemella sp | 0 | 1 | n/a |
Aerococcus sp | 1 | 0 | n/a |
Gram Negative Organism | |||
Haemophilus sp | 1 | 6 | 1 |
Pseudomonas sp | 3 | 3 | n/a |
Escherichia coli | 1 | 3 | 1 |
Klebsiella sp | 0 | 1 | n/a |
Herbaspirillum sp | 0 | 2 | n/a |
Prevotella sp | 1 | 1 | n/a |
Aeromonas sp | 0 | 1 | n/a |
Haematobacter sp | 0 | 1 | n/a |
Neisseria sp | 0 | 1 | 0 |
Proteus sp | 0 | 1 | n/a |
Ureaplasma sp | 0 | 1 | 1 |
Achromobacter sp | 1 | 0 | n/a |
Paracoccus sp | 0 | 1 | n/a |
Bartonella sp | 0 | 0 | 1 |
Chlamydia sp | 0 | 0 | 1 |
Kingella kingae | 0 | 0 | 1 |
Anaerobes | |||
Fusobacterium sp | 0 | 3 | n/a |
Aggregatibacter sp | 0 | 1 | n/a |
Actinomyces | 1 | 0 | 0 |
Other | |||
Mycoplasma sp | 0 | 5 | 0 |
(Candida albicans) | 1 | 0 | n/a |
Mycobacterium sp | 0 | 0 | 3 |
Unidentified organism | 3 | 3 | n/a |
Mixed species | 4 | 10 | n/a |
Total positives | 51 | 78 | 43 |