Figure 3
From: Structural variability of dyads relates to calcium release in rat ventricular myocytes
Overview of electrophysiological experiments. A and B illustrate a combined whole-cell patch clamp and confocal fluorescence microscopy experiment in a typical CTR (left) and IMY myocyte (right). (A) The voltage stimulus (top traces) and the corresponding calcium current (bottom traces). (B) x-t line-scan confocal fluorescence images of calcium spikes recorded simultaneously with calcium currents in A. The numbered arrowheads point to the positions of the release sites generating calcium spikes shown in C. (C) The time-fluorescence profiles of the spikes indicated in B. Panels A, B, and C have the same time coordinates. (D) The latencies and amplitudes of the recorded calcium spikes from 15 CTR and 17 IMY myocytes. Distribution diagrams of latencies and amplitudes are shown as columns (top and right panels, respectively). Black and red mark CTR and IMY group data, respectively.
