Figure 4
From: Generation of a conditional transgenic mouse model expressing human Phospholipase A2 Receptor 1
Tg-hPLA2R1 derived MEFs senesce prematurely. (A) Indicated MEFs were seeded at the same cell density and were PFA-fixed and stained by crystal violet 9 days later (A) or analyzed for SA-β-Gal activity 7 days later (B). Representative photographs and percentage of stained cells (means +/− SD, and p-value using student t-test, ***P < 0.005) are shown. (C) Cells were methanol fixed (permeabilized conditions), or (D) fixed using PFA (non-permeabilized conditions), and (C,D) IF against PLA2R1 was performed. Nuclear counterstaining was performed using Hoechst dye.
