Figure 1

Experimental design of the attenuation-compensated Airy light sheet microscope, and Airy beam profiles. (a) Experiment setup with attenuation-compensation provided by the NDF. (b–d) show the 2 + 1D Airy light-sheet profiles attenuated by an absorbing NIR dye (2 mM) and visualized in fluorescein to show two-photon fluorescence with (b) no attenuation-compensation, (c) attenuation-compensated by the NDF, and (d) attenuation-compensated by the SLM (\(\sigma =0.5\)). (e) Normalized peak transverse intensity versus propagation distance corresponding to beam profiles in (b–d). CAM: Camera, CL: Cylindrical Lens, CPM: Cubic Phase Mask, GM: Galvo Mirror, L: Lens, NDF: Neutral Density Filter, O: Objective, SC: Sample Chamber, SLM: Spatial Light Modulator, TL: Tube Lens. The \(x,y,z\) coordinate system shown in (a) applies within the sample volume only.