Figure 2 | Scientific Reports

Figure 2

From: Autologous fecal transplantation from a lean state potentiates caloric restriction effects on body weight and adiposity in obese mice

Figure 2

Effects of FMT on lipolysis in adipose tissue. Effects of FMT on (A) mRNA levels of ATGL coactivator CGI58, (B) total ATGL protein levels, (C) total HSL protein levels and (D, E, F) HSL phosphorylation in mesenteric fat from control and HFD mice. Effects of FMT on (G) mRNA levels of ATGL coactivator CGI58, (H) total ATGL protein levels, (I) total HSL protein levels and (J, K, L) HSL phosphorylation in retroperitoneal fat from control and HFD mice. Blots presented in this figure come from the same part of the same gel/blot. Full-length gels are provided as supplementary figures (Supplementary Figures 6-10). To achieve the number of samples needed for statistical analyses (n = 4), two gels/blots were used and were processed in parallel. Loading control (Ponceau S) has been run in the same blot to normalize the results. Data are expressed as mean ± SEM of 4 animals per group in the western-blot analyses while all animals were included for the quantification of CGI58 mRNA levels (at least 8 animals per group). Data are presented in comparison to the controls considered as 1. *p < 0.05, **p < 0.01, vs Control; #p < 0.05, ##p < 0.01, vs HFD, ap < 0.05, aap < 0.01 vs CR, $$p < 0.01 vs FT-H.

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