Figure 5

Pretreatment with 5,6-EET increased the adhesiveness of HCAECs to THP-1 cells. HCAECs were untreated (A) or treated with vehicle (B), 0.25 nM (C), 2.5 nM (D), or 25 nM (E) 5,6-EET for 24 h. The amount of THP-1 cell adherence is represented as relative fluorescence units. Pretreatment with 0.25 nM, 2.5 nM, or 25 nM 5,6-EET significantly and dose-dependently increased the adhesiveness of HCAECs to THP-1 cells (P < 0.001) (F).