Figure 4 | Scientific Reports

Figure 4

From: Functional analysis of an essential Ran-binding protein gene, CpRbp1, from the chestnut blight fungus Cryphonectria parasitica using heterokaryon rescue

Figure 4

Schematic representation of the complementing vectors and expected outcomes of complementation. (a) Full-length genomic DNA of the CpRbp1 gene was used as a control; vertical dashed lines in the genomic DNA represent introns. (b–g) A series of vector constructs containing various domains of CpRBP1 were constructed using cDNA of the CpRbp1 gene and an expression cassette consisting of a strong constitutive cryparin promoter (p188) and trpC terminator. (h) A heterologous gene from A. nidulans was analyzed using the corresponding cDNA and the expression cassette. Functional domains including the N-terminal region, RBD, and C-terminal region are indicated as boxes marked with N, RBD, and C, respectively. The NLS within the N-terminal region and the coiled-coil domain within the C-terminal region are indicated by dashed and dotted boxes, respectively. The promoter and terminator are indicated by a grey arrow and solid line, respectively. Dotted lines represent deleted amino acid residues. Numbers indicate amino acid residues of CpRBP1. (i) Schematic diagram of possible outcomes of complementation with functional genes.

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