Figure 5 | Scientific Reports

Figure 5

From: Alteration of Protein Binding Affinities by Aqueous Two-Phase Systems Revealed by Pressure Perturbation

Figure 5

Binding isotherms for ANS-BSA in buffer (A) and in the ATPS (B) at 1, 500, 1000, 1500 and 2000 bar. The best fits of experimental data according to an equivalent and independent binding sites model (A) or two classes of non-equivalent and independent binding sites model (B) are represented by the continuous coloured lines. All the experiments were carried out in 10 mM Tris buffer, pH 7.4 at T = 25 °C. For comparison, the binding isotherms were normalized.

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