Figure 6

Pressure dependence of the binding constants, Kbi, in buffer (black squares) and the ATPS (red circles for Kb1 and blue triangles for Kb2). From the slope of the Kb(p) data, the binding volumes, ΔVb, can be determined. In this expanded scale, the error bars seem to be large, but the actual volume changes and the corresponding error bars are very small, in order of 1–2 water molecules for ΔVb and a fraction of that for the error in ΔVb, only.