Figure 1

(a) Generation of artificial pools of RNA quasispecies or virus quasispecies. (left) Plasmids encoding PR or RT gene segments were linearized, then mixed in different proportions to generate artificial pools. RNA quasispecies was generated by in vitro transcription. (right) Viral particles were mixed in different proportions to generate virus quasispecies, then viral RNA was extracted. (b) The frequency of each RNA quasispecies was quantified using single variant sequencing, leveraging the primer-ID approach and high-throughput Illumina sequencing.