Figure 2
Background error rate of the single variant sequencing (SVS) method. Purified plasmids (in triplicate) or viruses (in duplicate) were subjected to the SVS analysis. For each of the three plasmids, RNA encoding HIV-1 PR (amino acids 8–99) or RT (amino acids 11–133) was generated by in vitro transcription, followed by the SVS procedure and Illumina sequencing. For each of the three virus stocks, viral RNA was extracted, then subjected to SVS and Illumina sequencing of the PR gene segment (amino acids 8–99). Frequency of amino acids called erroneously at all sequenced positions was scored and plotted as % mean error per position for the sequenced gene. Mean with SEM is shown.
