Figure 1

DG neurogenesis is attenuated in cyclin D2^−/− animals compared to cyclin D2^+/+ (WT) controls regardless of behavioral experience. (A), Representative micrographs of WT and cyclin D2^−/− males in grayscale Ki67 (yellow), DCX (red), and DAPI (cyan) followed by a merge of all 3 channels at 20X magnification. Dashed lines indicate the outer boundary of the GCL; scale bars represent 200 μm. Total DG estimates of (B), Ki67⁺ cells, (C), DCX⁺ cells, and (D), Ki67⁺/DCX⁺ cells between WT (gray circles) and cyclin D2^−/− (blue squares) genotypes. (E–G), There was a significant main effect of genotype on total DG cell count estimates from panels B–D stratified across behavioral groups (MWM and forced swim stress). There was no statistically significant difference in neurogenesis between animals that underwent either MWM or swim stress, but a significant main effect of genotype remained. There was no statistically significant effect of sex, and the data are therefore combined by sex. Data represent mean ± SEM. Statistical outcome: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.0001 via one-way ANOVA (B-D, combined behavioral groups: N = 20–24/genotype) or multivariate ANOVA (E-G, stratified behavioral groups: N = 10–12/genotype/behavioral group).