Figure 4
Plant growth stimulation of SAGs. (A) Main root length and (B) number of secondary roots of Arabidopsis plants grown on MS medium supplemented with SAGs (0.16 µg/ml) or naphthalene acetic acid (NAA), and control plants grown on non-supplemented medium was shown. Ten individual plants were analyzed per treatment, and the experiment was carried out three times. (C) Auxin-dependent DR5::GUS gene expression visualized as blue staining in Arabidopsis seedlings grown on SAGs (0.16 µg/ml) - or NAA-supplemented medium or on non-supplemented medium (control). Three independent experiments were performed with 10 plants per treatment. (D) Number of siliques of Arabidopsis plants, 5 weeks post-foliar treatment with SAGs (1.6 µg/ml) or water (Control). Three independent experiments with four random blocks of 6 plants per block were evaluated. (E) Effect of SAGs (50 µg/ml), BIAGRO10, SAGs + BIAGRO10, or water treatment (negative control) on number of pods in greenhouse-grown soybean plants. BIAGRO10 is a commercial bioinoculant based on the nitrogen fixing nodule-forming strain E109 of Bradyrhizobium japonicum. Four random blocks with 5 plants in each block were used in two independent experiments. Asterisks indicate statistically significant differences using DGC test (*p < 0.001, **p < 0.05).
