Figure 2

Interference study for determining cross-reactivity related to Brucella spp. with different concentration of WC mice IgG detection Ab and evaluation of effective working concentration of rabbit IgG capture Ab using developed S-ELISA. (a,b,c) Seven closely Brucella related bacterial spp. along with Brucella melitensis 16 M for S-ELISA against Brucella abortus S99 at 100, 50 and 25 µg mL⁻¹ of mice IgG detection Ab along x-axis. (d,e,f) Seven closely Brucella related bacterial spp. along with Brucella abortus S99 for S-ELISA against Brucella melitensis 16 M at 100, 50 and 25 µg mL⁻¹ of mice IgG detection Ab along x-axis. The y-axis showing absorbance at 495 nm respectively. (g,h) The WC rabbit IgG capture Ab at 10 and 20 µg mL⁻¹ concentration with 50 µg mL⁻¹ of mice IgG detection Ab against Brucella melitensis 16 M detecting 10 fold serial diluted (10⁸ CFU mL⁻¹ to 10¹ CFU mL⁻¹) WC Ag of Brucella melitensis 16 M along x-axis respectively. The y-axis showing absorbance at 495 nm. Polynomial regression co-efficient value for capture Ab at 10 µg mL⁻¹ is R² = 0.9431 with model equation, y = − 0.0121׳ + 0.2078ײ − 1.161×+ 2.3865 and for capture Ab at 20 µg mL⁻¹ concentration is R² = 0.9444 with model equation, y = − 0.0083׳ + 0.1399ײ − 0.7553×+ 1.5328 for Brucella WC detection.