Figure 5

SPR sensor response for interaction of immobilized mice IgG detection Ab with 10-fold serial diluted serum-spiked different concentrations of Brucella WC Ag. (a) Interaction of detection Ab with serum-spiked WC Ag of Brucella abortus S99 at (a) 1:5000 dilution of serum in PBS as an experimental control (b) 10² CFU mL⁻¹ (c) 10³ CFU mL⁻¹ (d) 10⁴ CFU mL⁻¹ (e) 10⁵ CFU mL⁻¹ (f) 10⁶ CFU mL⁻¹ and, (b) Interaction of detection Ab with serum-spiked WC Ag of Brucella melitensis 16 M, respectively at (a) 1:5000 dilution of serum in PBS as an experimental control. (b) 10² CFU mL⁻¹ (c) 10³ CFU mL⁻¹ (d) 10⁴ CFU mL⁻¹ (e) 10⁵ CFU mL⁻¹ (f) 10⁶ CFU mL⁻¹ in FBS:PBS dilution (1:5000) at RT (25 °C).