Figure 6

The representative ESR spectra for DMPO-OH generated by silver nitrate solution and UV-A irradiation with the dispersion of S. mutans containing the (a) cell wall, (b) cell membrane, and (c) cytoplasmic fraction, and the dispersion of A. actinomycetemcomitans containing the (d), cell wall, (e) cell membrane, and (f) cytoplasmic fraction. Spectra for (g) peptidoglycan (1 μg/mL) and (h) LPS (1 μg/mL). Since the ESR spectra generated from the cell membrane, cytoplasmic fraction and peptidoglycan were smaller than those generated from the cell wall, they are presented at higher magnification. Note that the difference in the MnO marker intensity. ○ and ▴ shows the MnO marker and DMPO-OH, respectively. The presence of DMPO-OH was confirmed by hyperfine coupling constants (hfcc) of aN = aH = 1.49 mT22.