Figure 4
Effect of altering temperatures on micellar behavior and cell injury induced by mixed micelles (9:1, STC:POPC, 1-palmitoyl, 2-oleyl phosphatidylcholine) in comparison to STC alone. (A) Thermograms representative of micellar breakdown at the indicated temperatures. (B) Enthalpograms representative of micellar breakdown of at the indicated temperatures. Data is from 3 experiments per condition (C) Critical micellar concentrations (dashed lines) measured as change in raw heat/ change in concentration (ΔQ/ΔC) at the indicated temperatures. Data is from n=3 experiments per condition (D) Cytosolic calcium increase induced by supra-micellar (10 mM) STC or STC + POPC at the indicated temperatures as measured using Fura-2 AM. Data is from 3 experiments per condition (E) Dose response curves comparing STC to STC + POPC in inducing cell injury at the indicated temperatures. Note that the injury per mole is higher at sub-micellar concentrations at 37 °C when the agents are monomeric, similar to what we note in Fig. 3D. *Indicated a p < 0.05 vs. 37C by one way ANOVA. Data is from 8 experiments per condition.
