Figure 6
Effect of pancreatic temperatures impairing micellar breakdown on serum bile acids, cardiac injury, function, shock and mortality: (A–F) Samples form Control rats (Con), those with STC pancreatitis (S), STC pancreatitis with balloon and no cooling (S + B) and STC pancreatitis with cooling (S + C) were collected at the time of euthanasia. (A) Plasma bile acids, (B) CK-MB (myocardial injury marker) (C) Representative images showing fluorescent PI uptake (administered 5 hours into pancreatitis or when pulse distention was less than 200 μm) by heart muscle nuclei (yellow arrows). Nuclei are stained blue with DAPI. Nonspecific red fluorescence outside nuclei is from RBCs. Changes from baseline in end-diastolic volume (ΔEDV; D) and stroke volume (ΔSV, E) after 3 hours of pancreatitis in the STC vs. STC+ cooling groups. (F) Carotid pulse distention measured 5 hours after inducing pancreatitis. (G) survival curves in different pancreatitis groups. Echocardiography mages of the left ventricles of rats with STC pancreatitis (H), and STC+ cooling (I) after 3 hours, showing short axis views of the left ventricle in diastole (upper panel) and M-mode images lower panel. These were taken at the same frame size. Note the lower diastolic expansion in (H).
